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Proteomic and phosphoproteomic comparison of human ES and iPS cells

renchunxiao 添加于 2012/1/31 14:17:35  1503次阅读 | 0次推荐 | 0个评论

Combining high-mass-accuracy mass spectrometry, isobaric tagging and software for multiplexed, large-scale protein quantification, we report deep proteomic coverage of four human embryonic stem cell and four induced pluripotent stem cell lines in biological triplicate. This 24-sample comparison resulted in a very large set of identified proteins and phosphorylation sites in pluripotent cells. The statistical analysis afforded by our approach revealed subtle but reproducible differences in protein expression and protein phosphorylation between embryonic stem cells and induced pluripotent cells. Merging these results with RNA-seq analysis data, we found functionally related differences across each tier of regulation. We also introduce the Stem Cell–Omics Repository (SCOR), a resource to collate and display quantitative information across multiple planes of measurement, including mRNA, protein and post-translational modifications.

作 者:Douglas H Phanstiel; Justin Brumbaugh; Craig D Wenger; Shulan Tian; Mitchell D Probasco; Derek J Bailey; Danielle L Swaney; Mark A Tervo; Jennifer M Bolin; Victor Ruotti; Ron Stewart; James A Thomson; Joshua J Coon
期刊名称: Nature Methods
期卷页: 2011-09-11 第8卷 第10期 821~827页
学科领域:生命科学 » 细胞生物学 » 细胞增殖、生长与分化
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原文链接:http://www.nature.com/nmeth/journal/v8/n10/abs/nmeth.1699.html
DOI: doi:10.1038/nmeth.1699
ISBN: 1548-7091
关键词: Proteomics, Stem Cells, Cell Biology, Mass Spectrometry
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dujuan123du
谢谢了!哦哦
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