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Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions

SprMorn 添加于 2011/12/8 16:22:03  2175次阅读 | 2次推荐 | 3个评论

Background aims. Human embryonic stem (hES) cells hold great potential for cell therapy and regenerative medicine because of their pluripotency and capacity for self-renewal. The conditions used to derive and culture hES cells vary between and within laboratories depending on the desired use of the cells. Until recently, stem cell culture has been carried out using feeder cells, and culture media, that contain animal products. Recent advances in technology have opened up the possibility of both xeno-free and feeder-free culture of stem cells, essential conditions for the use of stem cells for clinical purposes. To date, however, there has been limited success in achieving this aim. Methods, results and conclusions. Protocols were developed for the successful derivation of two normal and three specific mutation-carrying (SMC) (Huntington's disease and myotonic dystrophy 1) genomically stable hES cell lines, and their adaptation to feeder-free culture, all under xeno-free conditions.

作 者:Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions
期刊名称: Cytotherapy
期卷页: 第卷 第期 页
学科领域:生命科学 » 细胞生物学 » 细胞、亚细胞结构与功能
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原文链接:http://informahealthcare.com/doi/abs/10.3109/14653249.2011.623692?prevSearch=allfield%253A%2528Peter%2BBraude%2529&searchHistoryKey=
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相关报道: http://paper.sciencenet.cn/htmlpaper/201112816275012521031.shtm
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