首页>>文献首页>>文献正文

Mapping nanomechanical properties of live cells using multi-harmonic atomic force microscopy

renchunxiao 添加于 2011/11/22 15:13:40  1776次阅读 | 1次推荐 | 0个评论

The nanomechanical properties of living cells, such as their surface elastic response and adhesion, have important roles in cellular processes such as morphogenesis1, mechano-transduction2, focal adhesion3, motility4, 5, metastasis6 and drug delivery7, 8, 9, 10. Techniques based on quasi-static atomic force microscopy techniques11, 12, 13, 14, 15, 16, 17 can map these properties, but they lack the spatial and temporal resolution that is needed to observe many of the relevant details. Here, we present a dynamic atomic force microscopy18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28 method to map quantitatively the nanomechanical properties of live cells with a throughput (measured in pixels/minute) that is ~10–1,000 times higher than that achieved with quasi-static atomic force microscopy techniques. The local properties of a cell are derived from the 0th, 1st and 2nd harmonic components of the Fourier spectrum of the AFM cantilevers interacting with the cell surface. Local stiffness, stiffness gradient and the viscoelastic dissipation of live Escherichia coli bacteria, rat fibroblasts and human red blood cells were all mapped in buffer solutions. Our method is compatible with commercial atomic force microscopes and could be used to analyse mechanical changes in tumours, cells and biofilm formation with sub-10 nm detail.

文献笔记

   
导出选项:

评论 (0 个评论)

Page 1 of 1
<<<[1]>>>
发表评论:
验证码  
 

举报 | Archiver | 科学网( 京ICP备07017567 )

GMT+8, 2011-2-15 11:31

Powered by ScienceNet.cn

Copyright © 2007-2011 科学时报社