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In vitro assessment of human endothelial cell permeability: effects of inflammatory cytokines and dengue virus infection

leocane 添加于 2011/3/31 16:09:09  1161次阅读 | 1次推荐 | 4个评论

Electrical resistance across human umbilical vein endothelial cells (HUVECs) was measured using an electrical cell sensor system. The transendothelial electrical resistance (TEER) value was used to estimate the permeability through endothelial cells in vitro. Decrease in the TEER value was associated with increase in the passage of albumin through endothelial cells in the albumin permeability assay. The effects of cytokines and dengue virus infection on the permeability of HUVECs were examined by measuring the TEER value. Tumor necrosis factor alpha (TNF-α) at 1 and 0.1 μg/ml decreased the TEER value, but TNF-α at lower dose did not. Interferon-gamma (IFN-γ) at 1 μg/ml also decreased the TEER value. In contrast, interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10) or interferon-beta (IFN-β) did not decrease the TEER value. The decrease in the TEER value was associated with the morphological changes of HUVECs. Dengue virus infection at a multiplicities of infection (m.o.i.) of 5 pfu/cell decreased the TEER value. Infection at an m.o.i. of 0.5 pfu/cell did not decrease the TEER value; however, addition of 0.01 μg/ml of TNF-α to these infected endothelial cells decreased the TEER value. The results suggest that TNF-α and dengue virus infection decrease synergistically the TEER value of endothelial cells. The TEER method is easy, reliable and can be applicable to further analysis of the increase in the permeability of endothelial cells in vitro induced by inflammatory cytokines and dengue virus infection.

作 者:Beti Ernawati Dewi; Tomohiko Takasaki ;  Ichiro Kurane  
期刊名称: Journal of Virological Methods-- 121 (2)
期卷页: 2004/11/01 第卷 第期 页
学科领域:医学科学 » 呼吸系统 »
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DOI: 10.1016/j.jviromet.2004.06.013
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文献笔记

本文研究了细胞因子和登革热病毒对内皮通透性的影响。其中,TNF-α(浓度为1 和 0.1μg/ml)可降低TEER,而低浓度的TNF-α没有此作用。(IFN-gama) at 1μg/ml亦可以降低TEER。相反,interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10) or interferon-beta(IFN-_)均不能降低TEER。TEER的下降伴随着内皮形态的改变。本研究也表明,TEER技术是研究细胞因子诱导内皮通透性变化的简便易行的技术手段。

   
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