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PCGF5 is required for neural differentiation of embryonic stem cells

tshuang319 添加于 2018/8/31 14:53:03  326次阅读 | 0次推荐 | 0个评论

Polycomb repressive complex 1 (PRC1) is an important regulator of gene expression and development. PRC1 contains the E3 ligases RING1A/B, which monoubiquitinate lysine 119 at histone H2A (H2AK119ub1), and has been sub-classified into six major complexes based on the presence of a PCGF subunit. Here, we report that PCGF5, one of six PCGF paralogs, is an important requirement in the differentiation of mouse embryonic stem cells (mESCs) towards a neural cell fate. Although PCGF5 is not required for mESC self-renewal, its loss blocks mESC neural differentiation by activating the SMAD2/TGF-β signaling pathway. PCGF5 loss-of-function impairs the reduction of H2AK119ub1 and H3K27me3 around neural specific genes and keeps them repressed. Our results suggest that PCGF5 might function as both a repressor for SMAD2/TGF-β signaling pathway and a facilitator for neural differentiation. Together, our findings reveal a critical context-specific function for PCGF5 in directing PRC1 to control cell fate.

作 者:Yao Mingze, Zhou Xueke, Zhou Jiajian, Gong Shixin, Hu Gongcheng, Li Jiao, Huang Kaimeng, Lai Ping, Shi Guang, Hutchins AP, Sun Hao, Wang Huating, Yao Hongjie.
期刊名称: Nature Communications
期卷页: 2018.5.15 第9卷 第期 ~页
学科领域:生命科学 » 细胞生物学 » 细胞增殖、生长与分化
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原文链接:https://www.nature.com/articles/s41467-018-03781-0
DOI: doi: 10.1038/s41467-018-03781-0
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