首页>>文献首页>>文献正文

Biosynthesis of the Antibiotic Nonribosomal Peptide Penicillin in Baker’s Yeast

zhpd55 添加于 2017/5/8 7:50:06  218次阅读 | 0次推荐 | 0个评论

Fungi are a valuable source of enzymatic diversity and therapeutic natural products including antibiotics. By taking genes from a filamentous fungus and directing their efficient expression and subcellular localisation, we here engineer the baker's yeast Saccharomyces cerevisiae to produce and secrete the antibiotic penicillin, a beta-lactam nonribosomal peptide. Using synthetic biology tools combined with long-read DNA sequencing, we optimise productivity by 50-fold to produce bioactive yields that allow spent S. cerevisiae growth media to have antibacterial action against Streptococcus bacteria. This work demonstrates that S. cerevisiae can be engineered to perform the complex biosynthesis of multicellular fungi, opening up the possibility of using yeast to accelerate rational engineering of nonribosomal peptide antibiotics.

作 者:Ali R Awan, View ORCID ProfileBenjamin A Blount, David J Bell, Jack C H Ho, Robert M McKiernan, View ORCID ProfileTom Ellis
期刊名称: bioRxiv preprint
期卷页: Posted September 15, 2016. 第卷 第期 页
学科领域:医学科学 » 药物学 » 微生物药物
添加人是否为作者:
原文链接:http://biorxiv.org/content/biorxiv/early/2016/09/15/075325.full.pdf
DOI: https://doi.org/10.1101/075325
ISBN:
关键词: Biosynthesis, Penicillin, Baker’s Yeast
全文地址: 下载
备 注: The synthetic biologists from Imperial College London have re-engineered yeast cells to manufacture the nonribosomal peptide antibiotic penicillin. In laboratory experiments, they were able to demonstrate that this yeast had antibacterial properties against streptococcus bacteria. The authors of the study, which is published today in the journal Nature Communications, say their new method demonstrates the effectiveness of using this kind of synthetic biology as a route for discovering new antibiotics. This could open up possibilities for using re-engineered yeast cells to develop new forms of antibiotics and anti-inflammatory drugs from the nonribosomal peptide family. Nonribosomal peptides are normally produced by bacteria and fungi, forming the basis of most antibiotics today. Pharmaceutical companies have long experimented with nonribosomal peptides to make conventional antibiotics. The rise of antimicrobial resistance means there is a need to use genetic engineering techniques to find a new range of antibiotics from bacteria and fungi. However, genetically engineering the more exotic fungi and bacteria- the ones likely to have antibacterial properties—is challenging because scientists don't have the right tools and they are difficult to grow in a lab environment, requiring special conditions.
文献笔记

 
导出选项:

评论 (0 个评论)

Page 1 of 1
<<<[1]>>>
发表评论:
验证码  
 

举报 | Archiver | 科学网( 京ICP备07017567 )

GMT+8, 2011-2-15 11:31

Powered by ScienceNet.cn

Copyright © 2007-2011 科学时报社