首页>>文献首页>>文献正文

A regulatory gene induces trichome formation and embryo lethality in tomato

wenjie99 添加于 2011/7/21 14:29:50  1642次阅读 | 0次推荐 | 0个评论

Trichomes are universal biological structures originating from the aerial epidermis, which serve as an excellent model tostudy plant differentiation at the cell level. Although the pathway regulating trichome formation in the Rosids has been wellcharacterized, only very recently a few genes were identified for trichome initiation in the Asterids. In this study, we clonedWoolly (Wo), essential for trichome formation in tomato. Transgenic experiments revealed that the woolly phenotype is caused by themutation in Wo which encodes a homeodomain protein containing a bZIP motif and a START domain. We identified three alleles of Wo and found that each allele contains a missense mutation, which respectively results in an amino acid substitution at theC terminus. Microarray and expression analysis showed that the expression of a B-type cyclin gene, SlCycB2, is possibly regulated by Wo, which also participates in trichome formation. Suppression of Wo or SlCycB2 expression by RNAi decreased the number of type I trichomes, and direct protein–protein interaction was detected betweenthem, implying that both proteins may work together in the regulation of this type of trichome formation. Cytological observationand Wo transcript analysis in the developing seeds showed that embryo development was also correlated with Wo.

作 者:Yang, Changxian; Li, Hanxia; Zhang, Junhong; Luo, Zhidan; Gong, Pengjuan; Zhang, Chanjuan; Li, Jinhua; Wang, Taotao; Zhang, Yuyang; Lu, Yong'en; Ye, Zhibiao
期刊名称: Proceedings of the National Academy of Sciences
期卷页: 07/19/2011 第108卷 第29期 11836~页
学科领域:生命科学 » 遗传学与生物信息学 » 基因表达调控与表观遗传学
添加人是否为作者:
原文链接:http://www.pnas.org/content/108/29/11836.abstract?sid=b98e0a28-e690-4ee6-a9b3-4b9efe3ab3d4
DOI: 10.1073/pnas.1100532108
ISBN: 0027-8424
关键词:
相关报道: http://paper.sciencenet.cn/htmlpaper/201172210274219718229.shtm
备 注:
文献笔记

   
导出选项:

评论 (0 个评论)

Page 1 of 1
<<<[1]>>>

举报 | Archiver | 科学网( 京ICP备07017567 )

GMT+8, 2011-2-15 11:31

Powered by ScienceNet.cn

Copyright © 2007- 科学时报社