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Structure and Mechanism of a Nitrate Transporter

renchunxiao 添加于 2013/4/8 13:45:58  1914次阅读 | 0次推荐 | 5个评论

The nitrate/nitrite transporters NarK and NarU play an important role in nitrogen homeostasis in bacteria and belong to the nitrate/nitrite porter family (NNP) of the major facilitator superfamily (MFS) fold. The structure and functional mechanism of NarK and NarU remain unknown. Here, we report the crystal structure of NarU at a resolution of 3.1 Å and systematic biochemical characterization. The two molecules of NarU in an asymmetric unit exhibit two distinct conformational states: occluded and partially inward-open. The substrate molecule nitrate appears to be coordinated by four highly conserved, charged, or polar amino acids. Structural and biochemical analyses allowed the identification of key amino acids that are involved in substrate gating and transport. The observed conformational differences of NarU, together with unique sequence features of the NNP family transporters, suggest a transport mechanism that might deviate from the canonical rocker-switch model.

作 者:Hanchi Yan, Weiyun Huang, Chuangye Yan, Xinqi Gong, Sirui Jiang, Yu Zhao, Jiawei Wang, Yigong Shi
期刊名称: cell reports
期卷页: 第卷 第期 页
学科领域:生命科学 » 生物物理、生物化学与分子生物学 » 生物大分子结构与功能
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原文链接:http://www.cell.com/cell-reports/fulltext/S2211-1247(13)00114-9
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相关报道: http://paper.sciencenet.cn/htmlpaper/20134813484519928735.shtm
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发表评论人: [2015/4/16 15:26:34] 
thanks for share!
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