首页>>文献首页>>文献正文

Ubiquinone-quantum dot bioconjugates for in vitro and intracellular complex I sensing

AmandaPisces 添加于 2013/4/1 15:40:53  1115次阅读 | 0次推荐 | 0个评论

Quantum dots (QDs) have attracted increasing interest in bioimaging and sensing. Here, we report a biosensor of complex I using ubiquinone-terminated disulphides with different alkyl spacers (QnNS, n = 2, 5 and 10) as surface-capping ligands to functionalise CdSe/ZnS QDs. The enhancement or quenching of the QD bioconjugates fluorescence changes as a function of the redox state of QnNS, since QDs are highly sensitive to the electron-transfer processes. The bioconjugated QnNS-QDs emission could be modulated by complex I in the presence of NADH, which simulates an electron-transfer system part of the mitochondrial respiratory chain, providing an in vitro and intracellular complex I sensor. Epidemiological studies suggest that Parkinson's patients have the impaired activity of complex I in the electron-transfer chain of mitochondria. We have demonstrated that the QnNS-QDs system could aid in early stage Parkinson's disease diagnosis and progression monitoring by following different complex I levels in SH-SY5Y cells.

作 者:Wei Ma; Li-Xia Qin; Feng-Tao Liu; Zhen Gu; Jian Wang; Zhi Gang Pan; Tony D. James; Yi-Tao Long
期刊名称: Scientific Reports
期卷页: 2013-03-25 第3卷 第期 ~页
学科领域:生命科学 » 神经科学、认知科学与心理学 » 神经生物学
添加人是否为作者:
原文链接:http://www.nature.com/srep/2013/130325/srep01537/full/srep01537.html
DOI: doi:10.1038/srep01537
ISBN:
关键词:
备 注:
文献笔记

   
导出选项:

评论 (0 个评论)

Page 1 of 1
<<<[1]>>>

举报 | Archiver | 科学网( 京ICP备07017567 )

GMT+8, 2011-2-15 11:31

Powered by ScienceNet.cn

Copyright © 2007- 科学时报社