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Generation of functional eggs and sperm from cryopreserved whole testes

renchunxiao 添加于 2013/1/15 16:13:42  1328次阅读 | 0次推荐 | 0个评论

The conservation of endangered fish is of critical importance. Cryobanking could provide an effective backup measure for use in conjunction with the conservation of natural populations; however, methodology for cryopreservation of fish eggs and embryos has not yet been developed. The present study established a methodology capable of deriving functional eggs and sperm from frozen type A spermatogonia (ASGs). Whole testes taken from rainbow trout were slowly frozen in a cryomedium, and the viability of ASGs within these testes did not decrease over a 728-d freezing period. Frozen-thawed ASGs that were intraperitoneally transplanted into sterile triploid hatchlings migrated toward, and were incorporated into, recipient genital ridges. Transplantability of ASGs did not decrease after as much as 939 d of cryopreservation. Nearly half of triploid recipients produced functional eggs or sperm derived from the frozen ASGs and displayed high fecundity. Fertilization of resultant gametes resulted in the successful production of normal, frozen ASG-derived offspring. Feasibility and simplicity of this methodology will call for an immediate application for real conservation of endangered wild salmonids.

作 者:Seungki Lee, Yoshiko Iwasaki, Shinya Shikina, and Goro Yoshizaki1
期刊名称: Proceedings of the National Academy of Sciences
期卷页: 第卷 第期 ~页
学科领域:生命科学 » 动物学 » 动物生理及行为学
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原文链接:http://www.pnas.org/content/early/2013/01/09/1218468110.abstract?sid=07a2136e-b1b3-4862-84af-fc83c794ee6a
DOI: 10.1073/pnas.1218468110
ISBN: 0027-8424
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