首页>>文献首页>>文献正文

BRK1, a Bub1-Related Kinase, Is Essential for Generating Proper Tension between Homologous Kinetochores at Metaphase I of Rice Meiosis[W]

zhangxia111 添加于 2012/12/24 15:19:02  977次阅读 | 1次推荐 | 0个评论

Bub1 (for budding uninhibited by benzimidazole 1), one of the main spindle checkpoint kinases, acts as a kinetochore scaffold for assembling other checkpoint proteins. Here, we identify a plant Bub1-related kinase 1 (BRK1) in rice (Oryza sativa). The brk1 mutants are sterile due to the precocious separation of sister chromatids at the onset of anaphase I. The centromeric recruitment of SHUGOSHIN1 and phosphorylation of histone H2A at Thr-134 (H2A-pT134) depend on BRK1. Although the homologs can faithfully separate from each other at the end of meiosis I, the uncorrected merotelic attachment of paired sister kinetochores at the early stage of metaphase I in brk1 reduces the tension across homologous kinetochores, causes the metaphase I spindle to be aberrantly shaped, and subsequently affects the synchronicity of homolog separation at the onset of anaphase I. In addition, the phosphorylation of inner centromeric histone H3 at Ser-10 (H3-pS10) during diakinesis depends on BRK1. Therefore, we speculate that BRK1 may be required for normal localization of Aurora kinase before the onset of metaphase I, which is responsible for correcting the merotelic attachment.

作 者:Mo Wang, Ding Tang, Qiong Luo, Yi Jin, Yi Shen, Kejian Wang and Zhukuan Cheng
期刊名称:
期卷页: 第卷 第期 页
学科领域:生命科学 » 细胞生物学 » 细胞生长与分裂
添加人是否为作者:
原文链接:http://www.plantcell.org/content/early/2012/12/21/tpc.112.105874.abstract?sid=8a09c410-4d2c-4d65-8ca5-2c06f09de4cc
DOI:
ISBN:
关键词:
备 注:
文献笔记

   
导出选项:

评论 (0 个评论)

Page 1 of 1
<<<[1]>>>
发表评论:
验证码  
 

举报 | Archiver | 科学网( 京ICP备07017567 )

GMT+8, 2011-2-15 11:31

Powered by ScienceNet.cn

Copyright © 2007-2011 科学时报社