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Bioenergy and BiofuelsThermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing

zhangxia111 添加于 2012/11/6 11:22:26  2915次阅读 | 0次推荐 | 3个评论

Thermotolerant inulin-utilizing yeast strains are desirable for ethanol production from Jerusalem artichoke tubers by consolidatedbioprocessing (CBP). To obtain such strains, 21 naturally occurring yeast strains isolated by using an enrichment method and65 previously isolated Saccharomyces cerevisiae strains were investigated in inulin utilization, extracellular inulinase activity, and ethanol fermentation from inulin andJerusalem artichoke tuber flour at 40 掳C. The strains Kluyveromyces marxianus PT-1 (CGMCC AS2.4515) and S. cerevisiae JZ1C (CGMCC AS2.3878) presented the highest extracellular inulinase activity and ethanol yield in this study. The highestethanol concentration in Jerusalem artichoke tuber flour fermentation (200 gL1) at 40 掳C achieved by K. marxianus PT-1 and S. cerevisiae JZ1C was 73.6 and 65.2 gL1, which corresponded to the theoretical ethanol yield of 90.0 and 79.7 %, respectively. In the range of 30 to 40 掳C, temperaturedid not have a significant effect on ethanol production for both strains. This study displayed the distinctive superiorityof K. marxianus PT-1 and S. cerevisiae JZ1C in the thermotolerance and utilization of inulin-type oligosaccharides reserved in Jerusalem artichoke tubers. It isproposed that both K. marxianus and S. cerevisiae have considerable potential in ethanol production from Jerusalem artichoke tubers by a high temperature CBP.

作 者:Nan Hu, Bo Yuan, Juan Sun, Shi-An Wang and Fu-Li Li
期刊名称: Applied Microbiology and Biotechnology
期卷页: 第 95卷 第期 1359-1368页
学科领域:化学科学 » 有机化学 » 有机合成
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原文链接:http://www.springerlink.com/content/mm26x2v824wl17n6/
DOI: 10.1007/s00253-012-4240-8
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相关报道: http://paper.sciencenet.cn/htmlpaper/201211810494072626419.shtm
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