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Molecular targets of 14-alpha-lipoyl andrographolide on quorum sensing in Pseudomonas aeruginosa.

zhangxia111 添加于 2012/10/18 16:42:42  1031次阅读 | 0次推荐 | 1个评论

In Pseudomonas aeruginosa (P. aeruginosa), quorum sensing (QS) system is closely related to the biofilm formation. We previously demonstrated that 14-Alpha-lipoyl andrographolide (AL-1) has synergistic effects on antibiofilm and antivirulence factors (pyocyanin and exopolysaccharide) of P. aeruginosa when combined with conventional antibiotics, while has little inhibitory effect on its growth. However, its molecular mechanism remains elusive. Here we investigated the effect of AL-1 on QS, especially the Las and Rhl system. It showed that AL-1 can inhibit LasR-3-oxo-C(12)-HSL interactions and repress the transcriptional level of QS-regulated genes. RT-PCR data showed that AL-1 significantly reduced the expression of lasR, lasI, rhlR, and rhlI, in a dose-dependent manner. AL-1 not only decreased the expression of Psl which is positively regulated by Las system, but also increased the secretion of ExoS which is negatively regulated by Rhl system, indicating that AL-1 has multiple effects on both Las and Rhl system. It is no wonder that AL-1 showed synergistic effects with other antimicrobial agents in the treatment of P. aeruginosa infections.

作 者:Ma Li L,Liu Xiangyang X,Liang Haihua H,Che Yizhou Y,Chen Caixia C,Dai Huanqin H,Yu Ke K,Liu Mei M,Ma Luyan L,Yang Ching-Hong CH,Song Fuhang F,Wang Yuqiang Y,Zhang Lixin L,
期刊名称: Antimicrobial agents and chemotherapy
期卷页: 2012Jul16 第卷 第期 页
学科领域:生命科学 » 微生物学 » 病毒学
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原文链接:http://www.ncbi.nlm.nih.gov/pubmed/22802260
DOI: 10.1128/AAC.01119-12
ISBN: 1098-6596
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相关报道: http://paper.sciencenet.cn/htmlpaper/2012102611104264826121.shtm
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