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Nitrification of archaeal ammonia oxidizers in acid soils is supported by hydrolysis of urea

wrys 添加于 2012/8/29 11:10:32  1057次阅读 | 0次推荐 | 0个评论

The hydrolysis of urea as a source of ammonia has been proposed as a mechanism for the nitrification of ammonia-oxidizing bacteria (AOB) in acidic soil. The growth of Nitrososphaera viennensis on urea suggests that the ureolysis of ammonia-oxidizing archaea (AOA) might occur in natural environments. In this study, 15N isotope tracing indicates that ammonia oxidation occurred upon the addition of urea at a concentration similar to the in situ ammonium content of tea orchard soil (pH 3.75) and forest soil (pH 5.4) and was inhibited by acetylene. Nitrification activity was significantly stimulated by urea fertilization and coupled well with abundance changes in archaeal amoA genes in acidic soils. Pyrosequencing of 16S rRNA genes at whole microbial community level demonstrates the active growth of AOA in urea-amended soils. Molecular fingerprinting further shows that changes in denaturing gradient gel electrophoresis fingerprint patterns of archaeal amoA genes are paralleled by nitrification activity changes. However, bacterial amoA and 16S rRNA genes of AOB were not detected. The results strongly suggest that archaeal ammonia oxidation is supported by hydrolysis of urea and that AOA, from the marine Group 1.1a-associated lineage, dominate nitrification in two acidic soils tested.

作 者:Lu Lu, Wenyan Han, Jinbo Zhang, Yucheng Wu, Baozhan Wang, Xiangui Lin, Jianguo Zhu, Zucong Cai and Zhongjun Jia
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学科领域:地球科学 » 地质学 » 生物地质学
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原文链接:http://www.nature.com/ismej/journal/vaop/ncurrent/full/ismej201245a.html
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