首页>>文献首页>>文献正文

Induction of human neuronal cells by defined transcription factors

dankan 添加于 2011/6/1 16:37:17  1200次阅读 | 1次推荐 | 0个评论

Somatic cell nuclear transfer, cell fusion, or expression of lineage-specific factors have been shown to induce cell-fate changes in diverse somatic cell types1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12. We recently observed that forced expression of a combination of three transcription factors, Brn2 (also known as Pou3f2), Ascl1 and Myt1l, can efficiently convert mouse fibroblasts into functional induced neuronal (iN) cells13. Here we show that the same three factors can generate functional neurons from human pluripotent stem cells as early as 6 days after transgene activation. When combined with the basic helix–loop–helix transcription factor NeuroD1, these factors could also convert fetal and postnatal human fibroblasts into iN cells showing typical neuronal morphologies and expressing multiple neuronal markers, even after downregulation of the exogenous transcription factors. Importantly, the vast majority of human iN cells were able to generate action potentials and many matured to receive synaptic contacts when co-cultured with primary mouse cortical neurons. Our data demonstrate that non-neural human somatic cells, as well as pluripotent stem cells, can be converted directly into neurons by lineage-determining transcription factors. These methods may facilitate robust generation of patient-specific human neurons for in vitro disease modelling or future applications in regenerative medicine.

作 者:Zhiping P. Pang; Nan Yang; Thomas Vierbuchen; Austin Ostermeier; Daniel R. Fuentes; Troy Q. Yang; Ami Citri; Vittorio Sebastiano; Samuele Marro; Thomas C. Südhof; Marius Wernig
期刊名称: Nature
期卷页: 2011-05-26 第卷 第期 ~页
学科领域:生命科学 » 发育生物学与生殖生物学 » 发育生物学
添加人是否为作者:
原文链接:http://www.nature.com/nature/journal/vnfv/ncurrent/full/nature10202.html
DOI: doi:10.1038/nature10202
ISBN: 0028-0836
关键词: Stem cells, Neuroscience, Cell biology, Health and medicine
相关报道: http://paper.sciencenet.cn/htmlpaper/20116116383529917233.shtm
备 注:
来自论坛的回复:
文献笔记

   
导出选项:

评论 (0 个评论)

Page 1 of 1
<<<[1]>>>
发表评论:
验证码  
 

举报 | Archiver | 科学网( 京ICP备07017567 )

GMT+8, 2011-2-15 11:31

Powered by ScienceNet.cn

Copyright © 2007-2011 科学时报社